Article
A reduced folate carrier mutation produces substrate-dependent alterations in carrier mobility in murine leukemia cells and methotrexate resistance with conservation of growth in 5-formyltetrahydrofolate.
The Journal of biological chemistry - 3 Apr 1998
Zhao R, Assaraf Y G, Goldman I D
Abstract excerpt
With 5-formyltetrahydrofolate (5-CHO-THF) as the folate source a methotrexate (MTX) transport-deficient murine leukemia cell line, L1210-G1a, was isolated after chemical mutagenesis and MTX selection. This cell line was 10-fold resistant to MTX in comparison to parental L1210 cells, yet the EC50...
Topics
- Animals
- Antimetabolites, Antineoplastic
- Carrier Proteins
- Cell Division
- Drug Resistance, Neoplasm
- Folic Acid Antagonists
- Leucovorin
- Leukemia, Experimental
- Membrane Proteins
- Membrane Transport Proteins
- Methotrexate