Article
Discrimination among reduced folates and methotrexate as transport substrates by a phenylalanine substitution for serine within the predicted eighth transmembrane domain of the reduced folate carrier.
Biochemical pharmacology - 15 Nov 1999
Zhao R, Gao F, Goldman I D
Abstract excerpt
A phenylalanine substitution for serine in the reduced folate carrier at residue 309 (RFC1-S309F) was identified in a methotrexate (MTX)-resistant cell line selected with 5-formyltetrahydrofolate (5-CHO-THF) as the sole folate source. The transport characteristics of the mutated carrier were studied by transfection into the MTXrA line, which lacks endogenous RFC1 function. The level of expression of carrier in...
Topics
- Amino Acid Substitution
- Animals
- Antimetabolites, Antineoplastic
- Biological Transport
- Carrier Proteins
- Cell Division
- Chlorides
- DNA Mutational Analysis
- DNA, Complementary
- DNA, Neoplasm
- Drug Resistance, Neoplasm
- Kinetics
- Leucovorin