Article
Rapid haplotyping of mutations in the Duffy gene using the polymerase chain reaction and sequence-specific primers.
Tissue antigens - 1 Feb 1998
Mullighan C G, Marshall S E, Fanning G C, Briggs D C, Welsh K I
Abstract excerpt
The molecular basis for the antigenic variation and red cell expression of the Duffy antigen system has recently been elucidated. We have developed a simple one-step method for genotyping the single nucleotide polymorphisms in the promoter and exon of the Duffy gene using the polymerase chain reaction and sequence-specific primers (PCR-SSP). This method is also capable of haplotyping alleles at the two...
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