Article
An early transition state for folding of the P4-P6 RNA domain.
RNA (New York, N.Y.) - 1 Feb 2001
Silverman S K, Cech T R
Abstract excerpt
Tertiary folding of the 160-nt P4-P6 domain of the Tetrahymena group I intron RNA involves burying of substantial surface area, providing a model for the folding of other large RNA domains involved in catalysis. Stopped-flow fluorescence was used to monitor the Mg2+-induced tertiary folding of pyrene-labeled P4-P6. At 35 degrees C with [Mg2+] approximately 10 mM, P4-P6 folds on the tens of milliseconds timescale...
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