Hierarchy of base-pair preference in the binding domain of the bacteriophage T7 promoter.

doi:10.1006/jmbi.1993.1086

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Article

Hierarchy of base-pair preference in the binding domain of the bacteriophage T7 promoter.

Journal of molecular biology - 20 Feb 1993

Diaz G A, Raskin C A, McAllister W T

Abstract excerpt

The activity of bacteriophage T7 RNA polymerase (RNAP) at a collection of T7 promoter mutants having all possible single base-pair substitutions in the region from -15 to -6 was determined by transcription in vitro, thus establishing a hierarchy of base-pair preference at each position. The toler...

Topics

Bacteriophage T7
Base Composition
DNA, Viral
DNA-Directed RNA Polymerases
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
Promoter Regions, Genetic
Viral Proteins

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