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Article

Hierarchy of base-pair preference in the binding domain of the bacteriophage T7 promoter.

Journal of molecular biology - 20 Feb 1993

Diaz G A, Raskin C A, McAllister W T

Abstract excerpt

The activity of bacteriophage T7 RNA polymerase (RNAP) at a collection of T7 promoter mutants having all possible single base-pair substitutions in the region from -15 to -6 was determined by transcription in vitro, thus establishing a hierarchy of base-pair preference at each position. The tolerance of the RNAP for base-pair substitutions is not uniform across the binding domain. Under stringent conditions (20...

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Identifiers

PMID: 8445647
DOI: 10.1006/jmbi.1993.1086

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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