All 4 bases of both strands at -9 and -8 in T7 promoter are needed to be substituted by SP6-specific bases to switch promoter specificity.

Article

All 4 bases of both strands at -9 and -8 in T7 promoter are needed to be substituted by SP6-specific bases to switch promoter specificity.

Biochemistry and molecular biology international - 1 Dec 1993

Lee S S, Park S K, Cho I H, Kang C

Abstract excerpt

It has previously been shown that a substitution of two base pairs at -9 and -8 in T7 promoter by SP6 promoter-specific base pairs alone is sufficient to make the T7 variant become an SP6 promoter, abolishing T7 promoter activity [Lee, S. S., and Kang, C. (1992) Biochem. Int. 26, 1-5]. In order t...

Topics

Bacteriophage T7
Bacteriophages
Base Composition
DNA, Viral
DNA-Directed RNA Polymerases
Genetic Variation
Promoter Regions, Genetic
Transcription, Genetic

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