Article
A two-base-pair substitution in T7 promoter by SP6 promoter-specific base pairs alone abolishes T7 promoter activity but reveals SP6 promoter activity.
Biochemistry international - 1 Feb 1992
Lee S S, Kang C
Abstract excerpt
The phage T7 and SP6 RNA polymerase-promoter systems are very similar in many characteristics, but maintains stringent specificity for each. In order to identify the base pair element that distinguishes between T7 and SP6 promoters, the base pairs at -12, -10, -9, and -8 of the T7 promoter consensus sequence were changed singly and multiply to the SP6 promoter-specific base pairs, and assayed for T7 and SP6...
Topics
- Base Composition
- Base Sequence
- DNA-Directed RNA Polymerases
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- T-Phages
- Transcription, Genetic