Article
Metabolic consequences of phosphotransferase (PTS) mutation in a phenylalanine-producing recombinant Escherichia coli.
Biotechnology progress - 1 Jan 2000
Chen R, Hatzimanikatis V, Yap W M, Postma P W, Bailey J E
Abstract excerpt
E. coli strain PPA305, which has a wild-type PTS system, and PPA316, which utilizes a proton-galactose symport system for glucose uptake, were used as host strains to harbor a phenylalanine overproduction plasmid pSY130-14 and to study the effects of using different glucose uptake systems on phen...
Topics
- Adenosine Triphosphate
- Citric Acid Cycle
- Energy Metabolism
- Escherichia coli
- Fermentation
- Galactose
- Glucose
- Hydrogen-Ion Concentration
- Kinetics
- Magnetic Resonance Spectroscopy
- Mutation
- NADP
- Phenylalanine
- Phosphates
- Phosphoenolpyruvate
- Phosphotransferases
- Pyruvate Kinase
- Recombinant Proteins