Article
Characterization of growth and acid formation in a Bacillus subtilis pyruvate kinase mutant.
Applied and environmental microbiology - 1 Sept 2000
Fry B, Zhu T, Domach M M, Koepsel R R, Phalakornkule C, Ataai M M
Abstract excerpt
Based on measurements and theoretical analyses, we identified deletion of pyruvate kinase (PYK) activity as a possible route for elimination of acid formation in Bacillus subtilis cultures grown on glucose minimal media. Evidence consistent with the attenuation of PYK flux has come from metabolic flux calculations, metabolic pool and enzymatic activity measurements, and a series of nuclear magnetic resonance...
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