Article
Consequences of phosphoenolpyruvate:sugar phosphotranferase system and pyruvate kinase isozymes inactivation in central carbon metabolism flux distribution in Escherichia coli.
Microbial cell factories - 13 Sept 2012
Meza Eugenio, Becker Judith, Bolivar Francisco, Gosset Guillermo, Wittmann Christoph
Abstract excerpt
BACKGROUND: In Escherichia coli phosphoenolpyruvate (PEP) is a key central metabolism intermediate that participates in glucose transport, as precursor in several biosynthetic pathways and it is involved in allosteric regulation of glycolytic enzymes. In this work we generated W3110 derivative strains that lack the main PEP consumers PEP:sugar phosphotransferase system (PTS-) and pyruvate kinase isozymes PykA and...
Topics
- Amino Acids, Aromatic
- Biomass
- Carbon Cycle
- Escherichia coli
- Isoenzymes
- Mutation
- Phosphoenolpyruvate Carboxykinase (ATP)
- Phosphoenolpyruvate Carboxylase
- Phosphoenolpyruvate Sugar Phosphotransferase System
- Pyruvate Kinase