Article
The sacB gene cannot be used as a counter-selectable marker in Pasteurella multocida.
Molecular biotechnology - 1 Oct 1997
Jost B H, Homchampa P, Strugnell R A, Adler B
Abstract excerpt
The use of the Bacillus subtilis sacB gene as a counter-selectable marker was assessed in serogroup A and B strains of Pasteurella multocida. Expression ofsacB failed to render any of the strains sensitive to sucrose, indicating that the sacB gene can not be used as a positive selection system in P. multocida.
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