Article
MudSacI, a transposon with strong selectable and counterselectable markers: use for rapid mapping of chromosomal mutations in Salmonella typhimurium.
Journal of bacteriology - 1 Mar 1995
Lawes M, Maloy S
Abstract excerpt
The transposable bacteriophage Mu and its mini-Mu derivatives are useful tools for the genetic analysis of many bacteria. A variety of antibiotic-resistant Mu derivatives have been constructed, allowing direct selection for cells which contain the transposon. However, in many cases a counterselec...
Topics
- Bacteriophage mu
- Chromosome Mapping
- Chromosomes, Bacterial
- DNA Transposable Elements
- Genetic Complementation Test
- Genetic Markers
- Gram-Negative Bacteria
- Hexosyltransferases
- Mutagenesis, Insertional
- Mutation
- Restriction Mapping
- Salmonella typhimurium
- Selection, Genetic
- Sucrose