Article
Direct selection of cloned DNA in Bacillus subtilis based on sucrose-induced lethality.
Applied and environmental microbiology - 1 Nov 1996
Bramucci M G, Nagarajan V
Abstract excerpt
Expression of the Bacillus subtilis or Bacillus amyloliquefaciens sacB gene in the presence of sucrose is lethal for a variety of bacteria. Sucrose-induced lethality can be used to select for inactivation of sacB by insertion of heterologous DNA in sensitive bacteria. This procedure has not been...
Topics
- Bacillus
- Bacillus subtilis
- Base Sequence
- Cloning, Molecular
- DNA Primers
- DNA, Bacterial
- Gene Expression
- Gene Library
- Genes, Bacterial
- Hexosyltransferases
- Mutation
- Plasmids
- Sucrose