Article
An improved counterselection cassette for use in Haemophilus influenzae.
Gene - 15 Jan 2012
Johnston Jason W
Abstract excerpt
Counterselectable cassettes are extremely useful in molecular biology and allow for the creation of unmarked deletion mutants or the introduction of point mutations. I have constructed an inducible sacB cassette, using the tetracycline repressor. When used in tandem with a kanamycin-resistance marker, the cassette was successful in creating unmarked mutants in Haemophilus influenzae. The inducible nature of the...
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