Article
Generation of unmarked directed mutations in mycobacteria, using sucrose counter-selectable suicide vectors.
Molecular microbiology - 1 Jun 1996
Pelicic V, Reyrat J M, Gicquel B
Abstract excerpt
The expression of sacB, the Bacillus subtilis gene encoding levansucrase, is lethal to mycobacteria in the presence of 10% sucrose. In this study, we describe the use of sacB as a marker for positive selection of gene-replacement events into Mycobacterium smegmatis. A sucrose counter-selectable suicide plasmid was used to deliver an inactivated copy of the pyrF gene (pyrF::K(m)) into the M. smegmatis genome. Only...
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