Article
Generation of unmarked directed mutations in mycobacteria, using sucrose counter-selectable suicide vectors.
Molecular microbiology - 1 Jun 1996
Pelicic V, Reyrat J M, Gicquel B
Abstract excerpt
The expression of sacB, the Bacillus subtilis gene encoding levansucrase, is lethal to mycobacteria in the presence of 10% sucrose. In this study, we describe the use of sacB as a marker for positive selection of gene-replacement events into Mycobacterium smegmatis. A sucrose counter-selectable s...
Topics
- Alleles
- Genetic Vectors
- Hexosyltransferases
- Mutagenesis
- Mycobacterium
- Sucrose