Article
Positive selection of allelic exchange mutants in Mycobacterium bovis BCG.
FEMS microbiology letters - 1 Nov 1996
Pelicic V, Reyrat J M, Gicquel B
Abstract excerpt
sacB expression is lethal to mycobacteria in the presence of sucrose. It can therefore serve as 1 counter-selectable marker for positive selection of gene replacement events as demonstrated in the fast-growing Mycobacterium smegmatis. With this methodology, a sucrose counter-selectable vector was used to deliver, into the Mycobacterium bovis BCG genome, an inactivated copy (ureC::Km) of the ureC gene encoding the...
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