Article
Detection of Ki-ras mutations by PCR and differential hybridization and of p53 mutations by SSCP analysis in endoscopically obtained lavage solution from patients with long-standing ulcerative colitis.
The American journal of gastroenterology - 1 Dec 1997
Lang S M, Heinzlmann M, Stratakis D F, Teschauer W, Loeschke K
Abstract excerpt
OBJECTIVES: The goal of this study was the early detection of malignant transformation in patients with long-standing ulcerative colitis; therefore, mutations of the Ki-ras and p53 gene were analyzed in lavage solution and biopsies obtained at surveillance colonoscopy. METHODS: DNA was isolated f...
Topics
- Aspartic Acid
- Biopsy
- Case-Control Studies
- Cell Transformation, Neoplastic
- Codon
- Colitis, Ulcerative
- Colonic Neoplasms
- Colonoscopy
- DNA
- Digoxigenin
- Exons
- Female
- Genes, p53
- Genes, ras
- Genetic Testing
- Glycine
- Humans
- Immunoblotting