Identification of residues essential for differential fatty acyl specificity of Geotrichum candidum lipases I and II.

doi:10.1021/bi971390d

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Article

Identification of residues essential for differential fatty acyl specificity of Geotrichum candidum lipases I and II.

Biochemistry - 2 Dec 1997

Holmquist M, Tessier D C, Cygler M

Abstract excerpt

The fungus Geotrichum candidum produces two lipase isoenzymes, GCL I and GCL II, with distinct differences in substrate specificity despite their 86% identical primary structure. GCL I prefers ester substrates with long-chain cis (delta-9) unsaturated fatty acid moieties, whereas GCL II also acce...

Topics

Amino Acid Sequence
Caprylates
Genetic Variation
Geotrichum
Isoenzymes
Lipase
Molecular Sequence Data
Mutagenesis, Site-Directed
Oleic Acids
Pichia
Plasmids
Recombinant Proteins
Substrate Specificity
Triglycerides

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