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Article

Structure elucidation and docking analysis of 5M mutant of T1 lipase Geobacillus zalihae.

PloS one - 1 Jan 2021

Ishak Siti Nor Hasmah, Kamarudin Nor Hafizah Ahmad, Ali Mohd Shukuri Mohamad, Leow Adam Thean Chor, Shariff Fairolniza Mohd, Rahman Raja Noor Zaliha Raja Abd

Abstract excerpt

5M mutant lipase was derived through cumulative mutagenesis of amino acid residues (D43E/T118N/E226D/E250L/N304E) of T1 lipase from Geobacillus zalihae. A previous study revealed that cumulative mutations in 5M mutant lipase resulted in decreased thermostability compared to wild-type T1 lipase. Multiple amino acids substitution might cause structural destabilization due to negative cooperation. Hence, the...

Topics

Lipase
Geobacillus
Molecular Docking Simulation
Mutation
Hydrogen Bonding
Crystallography, X-Ray
Bacterial Proteins
Amino Acid Substitution
Enzyme Stability
Protein Conformation

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Identifiers

PMID: 34061877
DOI: 10.1371/journal.pone.0251751
PMCID: PMC8168862

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-34061877-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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