Article
Suppression of transformed phenotype and tumorigenicity after transfer of chromosome 4 into U251 human glioma cells.
Genes, chromosomes & cancer - 1 Nov 1997
Pershouse M A, Ligon A H, Pereira-Smith O M, Killary A M, Yung W K, Steck P A
Abstract excerpt
The development of primary human brain tumors, particularly glioblastoma multiforme (GBM), has been associated with a number of molecular and chromosomal abnormalities. In this study, a novel tumor suppressor locus was identified and localized after the transfer of a human chromosome 4 into U251 human GBM cells. Hybrid clones containing a transferred neomycin-resistance tagged chromosome 4 revealed an inability...
Topics
- Animals
- Blotting, Southern
- Chromosome Mapping
- Chromosomes, Human, Pair 4
- DNA
- Epidermal Growth Factor
- Genes, Tumor Suppressor
- Glioma
- Humans
- Hybrid Cells
- Mice
- Microsatellite Repeats
- Phenotype
- Polymerase Chain Reaction