Article
Glioblastoma-related gene mutations and over-expression of functional epidermal growth factor receptors in SKMG-3 glioma cells.
Acta neuropathologica - 1 Jun 2001
Thomas C, Ely G, James C D, Jenkins R, Kastan M, Jedlicka A, Burger P, Wharen R
Abstract excerpt
Amplification of the epidermal growth factor receptor (EGFR) gene is found in about 40% of glioblastomas (GBMs) but is rarely detected in GBM cell lines. We confirmed that the exceptional SKMG-3 GBM cell line retained amplified EGFR genes in vitro, and found that these sequences were concentrated on extra-chromosomal DNA particles similar to double-minute chromosomes. The cells contained two other gene mutations...
Topics
- Blotting, Southern
- Brain Neoplasms
- Cyclin-Dependent Kinases
- ErbB Receptors
- Flow Cytometry
- Gene Expression Regulation, Neoplastic
- Genes, p53
- Genotype
- Glioblastoma
- Humans
- In Situ Hybridization
- Mutation
- Phenotype
- Signal Transduction