Impact of mutations at the P4 and P5 positions on the reaction of antithrombin with thrombin and elastase.

doi:10.1016/s0049-3848(97)00228-4

Back to search

Article

Impact of mutations at the P4 and P5 positions on the reaction of antithrombin with thrombin and elastase.

Thrombosis research - 15 Oct 1997

Cunningham M A, Blajchman M A, Sheffield W P

Abstract excerpt

Antithrombin (AT) is a serpin capable of trapping thrombin (IIa) in a stable and covalent complex. Complex formation is prevented by leukocyte elastase (LE) cleavage near the AT reactive centre. We mutated the known LE cleavage sites of AT to explore the possibility of producing an LE-resistant A...

Topics

Amino Acid Substitution
Animals
Anticoagulants
Antithrombin III
Binding Sites
COS Cells
Drug Interactions
Genetic Variation
Humans
Leukocyte Elastase
Mutagenesis, Site-Directed
Rabbits
Thrombin

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.