Article
Kinetic characterization of the proteinase binding defect in a reactive site variant of the serpin, antithrombin. Role of the P1' residue in transition-state stabilization of antithrombin-proteinase complex formation.
The Journal of biological chemistry - 28 Apr 1995
Olson S T, Stephens A W, Hirs C H, Bock P E, Björk I
Abstract excerpt
To elucidate the role of the P1' residue of the serpin, antithrombin (AT), in proteinase inhibition, the source of the functional defect in a natural Ser-394-->Leu variant, AT-Denver, was investigated. AT-Denver inhibited thrombin, Factor IXa, plasmin, and Factor Xa with second order rate constan...
Topics
- Antithrombins
- Endopeptidases
- Enzyme Stability
- Humans
- Kinetics
- Leucine
- Mutation
- Protein Binding
- Serine