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Article

Engineering thrombin for selective specificity toward protein C and PAR1.

The Journal of biological chemistry - 18 Jun 2010

Marino Francesca, Pelc Leslie A, Vogt Austin, Gandhi Prafull S, Di Cera Enrico

Abstract excerpt

Thrombin elicits functional responses critical to blood homeostasis by interacting with diverse physiological substrates. Ala-scanning mutagenesis of 97 residues covering 53% of the solvent accessible surface area of the enzyme identifies Trp(215) as the single most important determinant of thrombin specificity. Saturation mutagenesis of Trp(215) produces constructs featuring k(cat)/K(m) values for the hydrolysis...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 20404340
DOI: 10.1074/jbc.M110.119875
PMCID: PMC2885193

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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