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Article

SV40 T-antigen is required for maintenance of immortal growth in SV40-transformed human fibroblasts.

Cell structure and function - 1 Feb 1991

Tsuyama N, Miura M, Kitahira M, Ishibashi S, Ide T

Abstract excerpt

Two lines of immortal human fibroblasts were isolated following transfection of TIG-3 cells with plasmid DNA, pMT-1ODtsA, that contained SV40 early gene with a deletion in replication origin and ts mutation in coding sequence for T-antigen. These cells continued proliferation at 34 degrees C, over 565 population doubling level (PDL) which is far over the limited division potential of untransformed normal TIG-3 of...

Topics

Antigens, Polyomavirus Transforming
Blotting, Southern
Cell Division
Cell Line, Transformed
Cell Transformation, Viral
Fibroblasts
Fluorescent Antibody Technique
Humans
Microscopy, Phase-Contrast
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Simian virus 40

Temperature

Transcription, Genetic

Transfection

Source

Identifiers

PMID: 1851674
DOI: 10.1247/csf.16.55

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1851674-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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SV40 T-antigen is required for maintenance of immortal growth in SV40-transformed human fibroblasts.PMID 1851674Read ↗

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