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Article

[Immortalization of human fibroblasts using tsA mutant of SV40 and pSV3neo plasmid].

Tsitologiia - 1 Jan 2001

Inozemtseva L S, Chernikov V G, Manuilova E S, Marshak M I, Nikolaeva N P, Kulagina O S, Grivennikov I A

Abstract excerpt

Clones of immortalized human fibroblasts with an extended life span in culture and a capability of subloning were obtained after the infection with a temperature sensitive mutant (tsA 239) of SV40 virus and pSV3neo plasmid. As compared with the parental cells, the obtained clones exhibited increased plating efficiency, decreased doubling time, and serum dependence. We did not obtained the colony formation during...

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PMID: 11769127

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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