Article
Two-step high resolution sequence-based HLA-DRB typing of exon 2 DNA with taxonomy-based sequence analysis allele assignment.
Human immunology - 1 Nov 2001
Luo M, Blanchard J, Brunham K, Pan Y, Shen C X, Lu H, Brunham R C
Abstract excerpt
A two-step high resolution sequence-based DRB typing method was developed. The system needs only one polymerase chain reaction (PCR) to type all functional DRB alleles of a given individual. It uses a pair of generic PCR primers to amplify exon 2 DNA of all functional DRB genes and a first-step taxonomy-based sequence analysis (FSTBSA) method to assign allele groups after sequencing the PCR products with a...
Topics
- Alleles
- Base Sequence
- DNA
- Exons
- HLA-DR Antigens
- HLA-DRB1 Chains
- HLA-DRB3 Chains
- HLA-DRB4 Chains
- HLA-DRB5 Chains
- Haplotypes
- Humans
- Linkage Disequilibrium
- Polymerase Chain Reaction