Article
HLA-DRB1 DNA sequencing based typing: an approach suitable for high throughput typing including unrelated bone marrow registry donors.
Tissue antigens - 1 Jan 2001
Sayer D, Whidborne R, Brestovac B, Trimboli F, Witt C, Christiansen F
Abstract excerpt
The HLA-DRB1 sequencing based typing strategies reported to date require separate amplifications of each sample with a series of group-specific primers followed by sequencing of any resulting polymerase chain reaction (PCR) products. Whilst this results in high resolution typing in the majority o...
Topics
- Alleles
- Bone Marrow Transplantation
- Family
- Gene Library
- HLA-DR Antigens
- HLA-DRB1 Chains
- Histocompatibility Testing
- Humans
- Polymerase Chain Reaction
- Registries
- Retrospective Studies
- Sensitivity and Specificity
- Sequence Analysis, DNA
- Tissue Donors