Article
Cells strongly expressing Ig(kappa) transgenes show clonal recruitment of hypermutation: a role for both MAR and the enhancers.
The EMBO journal - 1 Jul 1997
Goyenechea B, Klix N, Yélamos J, Williams G T, Riddell A, Neuberger M S, Milstein C
Abstract excerpt
The V regions of immunoglobulin kappa transgenes are targets for hypermutation in germinal centre B cells. We show by use of modified transgenes that the recruitment of hypermutation is substantially impaired by deletion of the nuclear matrix attachment region (MAR) which flanks the intron-enhanc...
Topics
- Animals
- B-Lymphocytes
- Binding Sites
- Enhancer Elements, Genetic
- Gene Deletion
- Gene Expression
- Immunoglobulin kappa-Chains
- Mice
- Mutation
- Nuclear Matrix
- Transgenes