Article
Monitoring and interpreting the intrinsic features of somatic hypermutation.
Immunological reviews - 1 Apr 1998
Neuberger M S, Ehrenstein M R, Klix N, Jolly C J, Yélamos J, Rada C, Milstein C
Abstract excerpt
We have used both normal and transgenic mice to analyse the recruitment and targeting of somatic hypermutation to the immunoglobulin loci. We compare methods for analysing hypermutation and discuss how large databases of mutations can be assembled by PCR amplification of the rearranged V-gene flanks from the germinal centre B cells of normal mice as well as by transgene-specific amplification from transgenic B...
Topics
- Animals
- Antibody Diversity
- Genes, Immunoglobulin
- Humans
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region
- Immunoglobulin kappa-Chains
- Mutation