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Article

Efficient expression and utilization of mutant 5 S rRNA in Saccharomyces cerevisiae.

The Journal of biological chemistry - 25 May 1990

Van Ryk D I, Lee Y, Nazar R N

Abstract excerpt

The expression of mutant 5 S rRNA genes in vivo is examined as a basis for further studies on the control, structure, and function of the ribosomal 5 S RNA. Specific single base substitutions (e.g. positions 98 or 99) or short insertions can result in substantial structural changes that can easily be detected by gel electrophoresis and permit the assay of mutant RNA synthesis and utilization. In addition, the use...

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PMID: 2187862

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