Article
Mutations in CR1 of E1A 12S yield dominant negative suppressors of immortalization and the lytic cycle.
Virology - 23 Jun 1997
Malladi A, Quinlan M P
Abstract excerpt
The Adenovirus 5 E1A 12S gene is responsible for the establishment of immortalization of primary cells by Adenovirus. We have identified two mutants of 12S (30K and NTdl814), which, when coexpressed with wild-type 12S in primary baby rat kidney cells, were capable of suppressing the immortalizing...
Topics
- Adenovirus E1A Proteins
- Animals
- Cells, Cultured
- Conserved Sequence
- Cytotoxins
- Exons
- Genetic Complementation Test
- Mutation
- Nuclear Proteins
- Nucleic Acid Synthesis Inhibitors
- Peptides
- Rats
- Repressor Proteins
- Retinoblastoma Protein
- Time Factors
- Trans-Activators
- Transcription Factors