Article
Immortalization of primary epithelial cells requires first- and second-exon functions of adenovirus type 5 12S.
Journal of virology - 1 Apr 1992
Quinlan M P, Douglas J L
Abstract excerpt
Immortalization of primary cells is a multistep process. The adenovirus E1A 12S gene product is a member of the class of oncoproteins that have the ability to establish primary cells as cell lines in culture. It is encoded by two exons. Extensive mutational analysis demonstrates that four regions of the E1A 12S gene, encoded by both exons, are necessary for immortalization of primary epithelial cells. Expression...
Topics
- Adenoviridae
- Adenovirus Early Proteins
- Animals
- Cell Cycle
- Cell Death
- Cell Differentiation
- Cell Division
- Cell Transformation, Viral
- Cells, Cultured
- Epithelial Cells
- Exons