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Article

Removal of polymerase-produced mutant sequences from PCR products.

Proceedings of the National Academy of Sciences of the United States of America - 24 Jun 1997

Smith J, Modrich P

Abstract excerpt

Heteroduplex DNA lacking d(GATC) methylation is subject to mismatch-provoked double-strand cleavage at d(GATC) sites in a reaction dependent on MutH, MutL, MutS, and ATP. We have exploited this reaction to develop a method for removal of polymerase-produced mutant sequences that arise during sequence amplification by PCR. After denaturation and reannealing, the PCR product pool is subjected to MutH, MutL, and...

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Share to Escherichia coli
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 9192654
DOI: 10.1073/pnas.94.13.6847
PMCID: PMC21247

Genes

GATC

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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