Article
Targeted nucleotide exchange in the alkaline phosphatase gene of HuH-7 cells mediated by a chimeric RNA/DNA oligonucleotide.
Hepatology (Baltimore, Md.) - 1 Jun 1997
Kren B T, Cole-Strauss A, Kmiec E B, Steer C J
Abstract excerpt
Although a variety of methods has been devised for modification of hepatic genes, none has been effective for long-term correction of genetic disorders. In this study, we employed a recently described novel experimental strategy for site-directed nucleotide exchange in genomic DNA of HuH-7 human hepatoma cells. A chimeric 2'-O-methylated-RNA/DNA oligonucleotide containing sequences complementary to 25 bases of...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.