Article
Isolation and characterization of human hepatoma cells with targeted insertions of a gpt selectable marker in the alpha 1-antitrypsin locus.
Somatic cell and molecular genetics - 1 Jul 1996
Porter M B, Fournier R E
Abstract excerpt
The bacterial xanthine-guanine phosphoribosyl transferase (gpt) gene was inserted by homologous recombination into the chromosomal alpha 1-antitrypsin (alpha 1AT) gene of HPRT-deficient human hepatoma cells. These insertions encoded chimeric alpha 1AT-gpt mRNAs that were expressed in the modified...
Topics
- Base Sequence
- Carcinoma, Hepatocellular
- DNA Primers
- DNA Transposable Elements
- DNA, Recombinant
- Genes, Bacterial
- Genetic Markers
- Genetic Vectors
- Genotype
- Humans
- Hybrid Cells
- Hypoxanthine Phosphoribosyltransferase
- In Situ Hybridization, Fluorescence
- Phenotype
- Recombination, Genetic
- Transfection
- Tumor Cells, Cultured
- alpha 1-Antitrypsin