Article
The association of nonsense mutation with exon-skipping in hprt mRNA of Chinese hamster ovary cells results from an artifact of RT-PCR.
RNA (New York, N.Y.) - 1 Jun 1997
Valentine C R, Heflich R H
Abstract excerpt
RT-PCR of RNA from CHO cells with nonsense mutations in the hprt gene frequently detects minor hprt mRNA species lacking one or more exons. Many nonsense mutants also contain greatly reduced concentrations of the major, normally spliced hprt mRNA. In this study, we examined the hypothesis that ex...
Topics
- Animals
- Artifacts
- CHO Cells
- Codon, Nonsense
- Cricetinae
- Exons
- Genes, Reporter
- Hypoxanthine Phosphoribosyltransferase
- Mutation
- Polymerase Chain Reaction
- RNA, Messenger
- Sequence Analysis, DNA
- Sequence Deletion
- Transcription, Genetic