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DNA sequence analysis of 1-nitrosopyrene-induced mutations in the hprt gene of Chinese hamster ovary cells.

Carcinogenesis - 1 May 1992

Newton R K, Mittelstaedt R A, Manjanatha M G, Heflich R H

Abstract excerpt

DNA sequence was determined in 21 mutants induced at the hprt locus of Chinese hamster ovary (CHO) cells by 1-nitrosopyrene, a metabolite of the tumorigenic environmental pollutant 1-nitropyrene. Following cDNA synthesis using RNA from each of the mutants, the hprt protein-coding region was amplified by the polymerase chain reaction (PCR) and subjected to direct DNA sequence analysis. Sixteen primary mutations...

Topics

Amino Acid Sequence
Animals
CHO Cells
Cricetinae
DNA
Hypoxanthine Phosphoribosyltransferase
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Pyrenes

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PMID: 1586993
DOI: 10.1093/carcin/13.5.819

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1586993-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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