Article
Recognition of AUG and alternative initiator codons is augmented by G in position +4 but is not generally affected by the nucleotides in positions +5 and +6.
The EMBO journal - 1 May 1997
Kozak M
Abstract excerpt
A primer extension (toeprinting) assay was used to monitor selection by ribosomes of the first versus the second AUG codon as a function of introducing mutations on the 3' side (positions +4, +5 and +6) of the first AUG codon. Six different flanking codons starting with G (GCG, GCU, GCC, GCA, GAU...
Topics
- Base Sequence
- Binding Sites
- Codon, Initiator
- Guanine
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Open Reading Frames
- Plasmids
- Protein Biosynthesis
- RNA Caps
- RNA, Messenger
- Structure-Activity Relationship