Article
Five-base codons for incorporation of nonnatural amino acids into proteins.
Nucleic acids research - 1 Sept 2001
Hohsaka T, Ashizuka Y, Murakami H, Sisido M
Abstract excerpt
Extension of the genetic code for the introduction of nonnatural amino acids into proteins was examined by using five-base codon-anticodon pairs. A streptavidin mRNA containing a CGGUA codon at the Tyr54 position and a tRNA(UACCG) chemically aminoacylated with a nonnatural amino acid were added to an Escherichia coli in vitro translation system. Western blot analysis indicated that the CGGUA codon is decoded by...
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