Article
Impact of natural IRS-1 mutations on insulin signals: mutations of IRS-1 in the PTB domain and near SH2 protein binding sites result in impaired function at different steps of IRS-1 signaling.
Diabetes - 1 Jun 1997
Yoshimura R, Araki E, Ura S, Todaka M, Tsuruzoe K, Furukawa N, Motoshima H, Yoshizato K, Kaneko K, Matsuda K, Kishikawa H, Shichiri M
Abstract excerpt
Insulin receptor substrate-1 (IRS-1) is one of the major substrates of insulin receptor tyrosine kinase and mediates various insulin signals downstream. In this study, we have examined the impact of three natural IRS-1 mutations identified in NIDDM patients (G971R, P170R, and M209T) on insulin si...
Topics
- Animals
- Blotting, Western
- Calcium-Calmodulin-Dependent Protein Kinases
- Cell Line
- DNA
- DNA, Complementary
- Electrophoresis, Polyacrylamide Gel
- Enzyme Activation
- Gene Expression Regulation
- Humans
- Insulin
- Insulin Receptor Substrate Proteins
- Mice