Article
The Gly-->Arg972 amino acid polymorphism in insulin receptor substrate-1 affects glucose metabolism in skeletal muscle cells.
The Journal of clinical endocrinology and metabolism - 1 May 2000
Hribal M L, Federici M, Porzio O, Lauro D, Borboni P, Accili D, Lauro R, Sesti G
Abstract excerpt
Molecular scanning of insulin receptor substrate-1 (IRS-1) revealed several amino acid substitutions. The most common IRS-1 variant, a Gly to Arg972 change, is more prevalent among type 2 diabetic patients. In this study we overexpressed wild-type and Arg972IRS-1 variant in L6 skeletal muscle cells and examined the functional consequences of this polymorphism on insulin metabolic signaling. L6 cells expressing...
Topics
- Amino Acid Substitution
- Animals
- Arginine
- Cell Line
- Cell Membrane
- Genetic Variation
- Glucose
- Glucose Transporter Type 1
- Glucose Transporter Type 4
- Glycine
- Glycogen
- Humans
- Insulin
- Insulin Receptor Substrate Proteins