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Article

Complementary strand analysis: a new approach for allelic separation in complex polyallelic genetic systems.

Nucleic acids research - 1 Jun 1997

Arguello R, Pay A L, McDermott A, Ross J, Dunn P, Avakian H, Little A M, Goldman J, Madrigal J A

Abstract excerpt

We describe a method, complementary strand analysis (CSA), for separating alleles potentially from any heterozygous genetic locus. Locus specific PCR is performed generating two allelic products. The antisense strands are isolated and hybridised with a sense reference strand to form a chimeric DNA duplex for each allele which is then separated by non-denaturing PAGE. We demonstrate the application of CSA for...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 9153329
DOI: 10.1093/nar/25.11.2236
PMCID: PMC146725

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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