Article
Complementary strand analysis: a new approach for allelic separation in complex polyallelic genetic systems.
Nucleic acids research - 1 Jun 1997
Arguello R, Pay A L, McDermott A, Ross J, Dunn P, Avakian H, Little A M, Goldman J, Madrigal J A
Abstract excerpt
We describe a method, complementary strand analysis (CSA), for separating alleles potentially from any heterozygous genetic locus. Locus specific PCR is performed generating two allelic products. The antisense strands are isolated and hybridised with a sense reference strand to form a chimeric DN...
Topics
- Alleles
- Electrophoresis, Polyacrylamide Gel
- Female
- Genetic Complementation Test
- Genetic Techniques
- HLA-A Antigens
- HLA-B Antigens
- HLA-C Antigens
- Humans
- Male
- Polymerase Chain Reaction
- Polymorphism, Genetic