HLA-DPB typing using co-digestion of amplified fragments allows efficient identification of heterozygous genotypes.

doi:10.1111/j.1399-0039.1992.tb02042.x

Back to search

Article

HLA-DPB typing using co-digestion of amplified fragments allows efficient identification of heterozygous genotypes.

Tissue antigens - 1 Oct 1992

Sawitzke A D, Sawitzke A L, Ward R H

Abstract excerpt

Twenty-four alleles have been defined for HLA-DPB based on their second exon sequences. This paper describes a novel method, co-digested amplified fragment length polymorphisms (CAFLP), for assigning these alleles to heterozygous patients, as well as to homozygous cell lines. The method depends o...

Topics

Adult
Alleles
Base Sequence
Child
Exons
Female
Gene Amplification
Genetic Carrier Screening
Genotype
HLA-DP Antigens
Haplotypes
Histocompatibility Testing
Humans
Male
Molecular Sequence Data
Polymorphism, Restriction Fragment Length

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.