Article
HLA-DPB typing using co-digestion of amplified fragments allows efficient identification of heterozygous genotypes.
Tissue antigens - 1 Oct 1992
Sawitzke A D, Sawitzke A L, Ward R H
Abstract excerpt
Twenty-four alleles have been defined for HLA-DPB based on their second exon sequences. This paper describes a novel method, co-digested amplified fragment length polymorphisms (CAFLP), for assigning these alleles to heterozygous patients, as well as to homozygous cell lines. The method depends on co-digestion of amplified DNA by restriction endonucleases and separation of the resultant fragments with...
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