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Article

Rapid cloning of HLA class I cDNAs by locus specific PCR.

Journal of immunological methods - 13 Jan 2000

Johnson D R, Biedermann B C, Mook-Kanamori B

Abstract excerpt

The human major histocompatibility complex (MHC) class I loci, human leukocyte antigen (HLA)-A, -B, -C, encode highly polymorphic molecules that mediate immune recognition of infectious pathogens and can initiate the rapid rejection of transplanted tissue. Cloning of HLA class I alleles is complicated by polymorphism as well as interlocus homology. Here, HLA class I cDNAs are amplified by PCR using one common...

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PMID: 10648862
DOI: 10.1016/s0022-1759(99)00121-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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