Sequencing-based typing of HLA-A locus using mRNA and a single locus-specific PCR followed by cycle-sequencing with AmpliTaq DNA polymerase, FS.

doi:10.1111/j.1399-0039.1997.tb02779.x

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Article

Sequencing-based typing of HLA-A locus using mRNA and a single locus-specific PCR followed by cycle-sequencing with AmpliTaq DNA polymerase, FS.

Tissue antigens - 1 May 1997

Norgaard L, Fugger L, Jakobsen B K, Svejgaard A

Abstract excerpt

The large number (59) of alleles now known at the HLA-A locus is a serious challenge to the existing methods for HLA typing, including many of the DNA based methods. Here, we describe a sequencing-based typing (SBT) protocol for typing of HLA-A alleles using a single A-locus-specific PCR. This re...

Topics

Alleles
Base Sequence
DNA-Directed DNA Polymerase
HLA-A Antigens
Histocompatibility Testing
Humans
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger
Sequence Analysis

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