Article
HLA class I sequence-based typing using DNA recovered from frozen plasma.
Journal of immunological methods - 31 Aug 2012
Cotton Laura A, Abdur Rahman Manal, Ng Carmond, Le Anh Q, Milloy M-J, Mo Theresa, Brumme Zabrina L
Abstract excerpt
We describe a rapid, reliable and cost-effective method for intermediate-to-high-resolution sequence-based HLA class I typing using frozen plasma as a source of genomic DNA. The plasma samples investigated had a median age of 8.5 years. Total nucleic acids were isolated from matched frozen PBMC (~2.5 million) and plasma (500 μl) samples from a panel of 25 individuals using commercial silica-based kits....
Topics
- Adolescent
- Adult
- Alleles
- Base Sequence
- Child
- Cryopreservation
- DNA
- Genes, MHC Class I
- Histocompatibility Testing
- Humans
- Leukocytes, Mononuclear
- Polymerase Chain Reaction