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Article

HLA class I sequence-based typing using DNA recovered from frozen plasma.

Journal of immunological methods - 31 Aug 2012

Cotton Laura A, Abdur Rahman Manal, Ng Carmond, Le Anh Q, Milloy M-J, Mo Theresa, Brumme Zabrina L

Abstract excerpt

We describe a rapid, reliable and cost-effective method for intermediate-to-high-resolution sequence-based HLA class I typing using frozen plasma as a source of genomic DNA. The plasma samples investigated had a median age of 8.5 years. Total nucleic acids were isolated from matched frozen PBMC (~2.5 million) and plasma (500 μl) samples from a panel of 25 individuals using commercial silica-based kits....

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PMID: 22584155
DOI: 10.1016/j.jim.2012.05.003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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