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Article

A high-throughput screen for MscL channel activity and mutational phenotyping.

Biochimica et biophysica acta - 1 Oct 2001

Maurer J A, Dougherty D A

Abstract excerpt

A novel fluorescence-based screen for bacterial mechanosensitive ion-channel activity has been developed. This assay is capable of clearly distinguishing the previously observed gain of function and loss of function phenotypes for the Escherichia coli mechanosensitive channel of large conductance (Ec-MscL). The method modifies Molecular Probes' Live/Dead BacLight bacterial viability assay to monitor MscL channel...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 11557017
DOI: 10.1016/s0005-2736(01)00390-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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A high-throughput screen for MscL channel activity and mutational phenotyping.PMID 11557017Read ↗

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Hydrophilicity of a single residue within MscL correlates with increased channel mechanosensitivity.Semantic proximity Read ↗

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