Article
Specificity of seven monoclonal antibodies against p53 evaluated with Western blotting, immunohistochemistry, confocal laser scanning microscopy, and flow cytometry.
Cytometry - 1 May 1997
Bonsing B A, Corver W E, Gorsira M C, van Vliet M, Oud P S, Cornelisse C J, Fleuren G J
Abstract excerpt
p53 immunostaining of histological sections shows inter- and intratumor variability in distribution and staining intensity which are usually scored semiquantitatively. In order to investigate the variation in p53 expression more accurately and its possible relation to other cellular parameters (e.g., DNA content), we have studied the possibility to measure p53 accumulation by multiparameter flow cytometry. To...
Topics
- Antibodies, Monoclonal
- Antibody Specificity
- Blotting, Western
- Breast Neoplasms
- DNA Mutational Analysis
- Female
- Flow Cytometry
- Fluorescent Antibody Technique, Indirect
- Genes, p53
- Humans