Article
Genetic and immunochemical analysis of mutant p53 in human breast cancer cell lines.
Oncogene - 1 Jun 1990
Bartek J, Iggo R, Gannon J, Lane D P
Abstract excerpt
The expression of the tumour suppressor gene p53 was analysed in 11 human breast cancer cell lines by immunohistochemistry, immunoprecipitation and cDNA sequencing. We used a panel of anti-p53 monoclonal antibodies for cell staining and found abnormalities in every case. Eight of the cell lines p...
Topics
- Amino Acid Sequence
- Antibodies
- Breast Neoplasms
- Carcinoma, Intraductal, Noninfiltrating
- DNA, Neoplasm
- Epitopes
- Gene Expression
- Humans
- Immunohistochemistry
- Molecular Sequence Data
- Mutation
- Oncogene Proteins
- Phosphoproteins
- Polymerase Chain Reaction
- Precipitin Tests
- RNA, Neoplasm
- Transcription, Genetic
- Tumor Cells, Cultured